In vitro cytotoxicity of self-curing acrylic resins of different colors

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Edition V19N04 | Year 2014 | Editorial Original Article | Pages 66 to 70

Luciana Borges Retamoso , Taís De Morais Alves Da Cunha , Matheus Melo Pithon , Rogério Lacerda Dos Santos , Fernanda Otaviano Martins , Maria Teresa Villela Romanos , Orlando Motohiro Tanaka

Objective: The aim of this study was to assess the in vitro cytotoxicity of acrylic resins of different colors over time. Methods: Specimens were divided into 4 groups (n = 6) according to the color of the acrylic resin (Orto Class, Clássico, Campinas, São Paulo, Brazil): Group 1: clear acrylic resin; group 2: pink acrylic resin; group 3: blue acrylic resin and group 4: green acrylic resin. All specimens were fabricated according to the mass manipulation technique and submitted to mechanical polishing protocol. The control was performed with an amalgam specimen (C+), a glass specimen (C-) and cell control (CC). Specimens were immersed in Minimum Eagle’s Medium (MEM) and incubated for 24 h at 37o C. The extracts from the experimental material were filtered and mixed with L929 fibroblast. Cytotoxicity was evaluated at 4 different times, 24, 48, 72 and 168 h. After contact, cells were incubated for 24 h and added to 100 µ of 0.01% neutral red dye. The cells were incubated for 3 h for pigment incorporation and fixed. Cells viability was determined by a spectroscopic (BioTek, Winooski, Vermont, USA) with a 492-nm wavelength λ=492 nm). Results: There were no statistical differences between the experimental groups and the CC and C- groups. Conclusion: Clear, pink, blue and green self-curing acrylic resins fabricated by means of the mass manipulation technique and mechanically polished are not cytotoxic. Neither the pigment added to the self-curing acrylic resin nor the factor of time influenced the cytotoxicity of the material.

Acrylic resins, Cell culture techniques, Cytotoxins,

Retamoso LB, Cunha TMA, Pithon MM, Santos RL, Martins FO, Romanos MTV, Tanaka OM. In vitro cytotoxicity of self-curing acrylic resins of different colors. Dental Press J Orthod. 2014 July-Aug;19(4):66-70. DOI:

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